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| Acetylgalactosaminidase 乙酰半乳糖胺酶 |
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Alpha-N-Acetylgalactosaminidase(Acremonium sp.) 100086
Endo-alpha-N-Acetylgalactosaminidase(Alcaligenes sp.)100453
Alpha-N-Acetylgalactosaminidase(Acremonium sp.)
alpha - N - Acetyl - D - galactosaminide
N - acetylgalactosaminohydrolase
( alpha - N - Acetyl - D - galactosaminidase)
- REACTION:
SUBSTRATES:
- Non - reduces termiinal alpha - N - acetylgalactosaminide
- REACTION SCHEMA:
- Hydrolizing cleavage of the substrate at non - reduced terminal alpha - N - Acetylgalactosaminide linkage
- REACTION PRODUCTS:
- alpha - N - acetylgalactosamine
- FORM:
- Solution in 10mM K - phosphate buffer (pH7.0) containing 20% glycerin
- ACTIVITY:
- 13units/ml (120units/mg protein)
- CONTAMINANTS:
- alpha - Galactosidase <0.15%
- PURITY:
- Homogeneous by PAGE
- OPTIMUM pH:
- 4.0 ~ 4.5
- OPTIMUM TEMP:
- 55oC
- M.W.:
- 55,000 (Gel filtration), 57,000 (SDS - PAGE)
- STORAGE:
- -20oC
- UNIT DEFINITION:
- One unit of enzyme catalyzes the release of 1 micro mole of p - nitrophenol from p - nitrophenyl - alpha -D - N - acetylgalactosaminide per minute at 37oC, pH4.5.
- ASSAY METHOD:
REACTION MIXTURE
- -Substrate:
- 2mM pNP - alpha - D - GalNAc 250 micro l
- 0.05M citrate buffer, pH4.5
- -Enzyme: 50 micro l
- REACTION
- -Incubation for 15 min. at 37oC
¥Ïl
- REACTION
- -Incubation for 15 min. at 37oC
Endo-alpha-N-Acetylgalactosaminidase(Alcaligenes sp.)
D - Galactosyl - N - acetamidodeoxy - alpha - D - galactoside - D - Galactosyl - N - acetamidodeoxy - D - galactohydrolase
- REACTION:
SUBSTRATES:
- 3GalNAc alpha - Ser(Thr) linkage in glycoproteins and glycopeptides
- REACTION SCHEMA:
- Hydrolizing cleavage of the substrate at alpha - N - acetylgalactoside - Ser (Thr) linkage on glycopeptides
- REACTION PRODUCTS:
- Gal beta 1 - 3GalNAc alpha
- FORM:
- Liquid
- ACTIVITY:
- 3 units/mg
- PURITY:
- Homogeneous by disc - PAGE
- OPTIMUM pH:
- 4.5
- OPTIMUM TEMP:
- 40oC
- M.W.:
- 160,000 (gel filtration or SDS - PAGE)
- STORAGE:
- -20oC
- UNIT DEFINITION:
- One unit of enzyme catalyzes the release of 1 micro mole of Gal - GalNAc from asialofetuin per minute at 37oC, pH4.5.
- ASSAY METHOD:
REACTION MIXTURE
- -Substrate:
- 0.4mM asialofetuin 40 micro l
- -Buffer:
- 0.2M citrate buffer, pH4.5
- -Enzyme: 0.2 - 1.8mu 30 micro l
- REACTION
- -Incubation for 10 min. at 37oC
- REFERENCES:
- 1) Agric. Biol. AChem. 51, 3169 (1987)
- 2) J. Biol. Chem., 217, 959 (1955)
Agric. Biol. AChem. 51, 3169 (1987)
2) J. Biol. Chem., 217, 959 (1955)
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