The cell walls of many genera of fungi contain significant levels of (1,3)-ß-D-Glucan, a pro-inflammatory material. This glucan is shed into the environment where fungi grow, and it may remain as an unwanted contaminant in air, water, and processed materials such as pharmaceuticals. Cellulosic materials may also leach (1,3)-ß-D-Glucan. The Glucatell kit is a (1,3)-ß-D-Glucan specific chromogenic reagent which is designed for glucan quantitation, using the familiar 96 well microplate format. The Glucatell kit is offered in a variety of kit formats, including kinetic and end-point diazo.  For serum analysis of (1,3)-ß-D-Glucan, see Fungitell® our new FDA-cleared diagnostic kit featured in the Clinical Diagnostic section of our website.

Glucatell Applications
LAL Endotoxin Out of Specification Results (OOS): Occasionally, LAL false positives results may be encountered. The resulting OOS investigation is made simpler if a root cause determination can be made. Glucatell can assist in verifying an alternative explanation for OOS LAL results, truly establishing the basis of a false positive.

Cellulose Filter Preparation: Experience has shown that cellulose filters may leach large quantities of (1,3)-ß-D-Glucan. Monitoring both filter rinse and final product for glucan contamination is easily accomplished with the Glucatell kit .

rDNA Yeast Protein Production: A popular host for recombinant protein production, yeast easily contaminate product with cell-derived glucan. The Glucatell kit may be used to monitor glucan levels through the purification process.

Cell Culture Fluid: Fungal (1,3)-ß-D-Glucan is a pro-inflammatory biological response modifier. Avoidance of extraneous, artifact-producing contaminants is a goal for both manufacturers and consumers.

Airborne Contamination: "Sick building syndrome," bad workplace air, and "moldy" environments may all have high levels of airborne glucan in common. This may be monitored using air sampling onto filters and the Glucatell kit .

Mycosis Research: Serum burdens of fungal (1,3)-ß-D-Glucan can be accurately and reproducibily monitored using Fungitell® .  This product is now featured in the Clinical Diagnostic section of our website.