| 产品目录 |
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| 酵母裂解酶Zymolyase |
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| 120491 |
ZymolyaseTM-20T (20 KU/mg) |
1 g
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| 120493 |
ZymolyaseTM-100T (100 KU/mg) |
500mg
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Zymolyase®-20T
(from Arthrobacter luteus)
Zymolyase®-20T, produced by a submerged culture of Arthrobacter luteus(1), is a new enzyme preparation which lyses effectively cell walls of viable yeast cells(2, 3). This preparation is a lyophilized powder prepared by salting out from the culture fluid with ammonium sulfate. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1, 3-linkages and releases specifically laminaripentaose as the main and minimum product unit(4, 5, 10, 11).
The extent of lysis of yeast cells by Zymolyase®-20T varies with yeast strain, growth stage of yeast, or cultural condition(6 - 8).
Zymolyase®-20T shows 20,000 units/g of the lytic activity, defined after, toward brewer's yeast cells (Saccharomyces cerevisiae, resting stage) or toward yeast cells of Saccharomyces cerevisiae IFO 0565 cultured statically in malt extract medium (Malt extract 2 g, peptone 0.5 g, water 100 ml) at 20oC for 34 hours.
Further purified preparation(9) is also available as Zymolyase® -100T whose specific activity is 100,000 units/g.
Further information related to the Zymolyase® are obtained in the references sited below(12 - 16).
- SPECIFICATIONS:
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| Appearance: |
Lyophilized powder |
| Activity: |
20,000 units/g |
| Essential enzyme: |
b - 1, 3 - glucan laminaripentaohydrolase |
| Other activities contained : |
- b - 1, 3 - glucanase
- protease
- mannanase
- (See reference No. 3 as to the definition of each enzyme unit. Each activity varies more of less among lots.)
- amylase, xylanase, phosphatase
- DNase, RNase
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- ca. 1.5 x 106 units/g
- ca. 1.5 x 104 units/g
- ca. 1.5 x 106 units/g
- tion of each enzyme unit. Each activity va
- minute amounts
- not detected
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| Optimum pH and temperature: |
pH 7.5, 35oC (for lysis of viable yeast cells) |
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pH 6.5, 45oC (for hydrolysis of yeast glucan) |
| Stable pH: |
5-10 |
| Heat stability: |
The lytic activity is lost on incubation at 60oC for 5 minutes. |
| Specificity (lytic spectrum)(5): |
Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloekera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomcopsis, Saccharomycodes, Schwanniomyces, etc. |
| Activators: |
SH compound such as cystein, 2-mercaptoethanol of dithiothreitol |
- ASSAY FOR ENZYME ACTIVITY:
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- Unit Definition
- One unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800 mm (A800) of the reaction mixture under the following condition.
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- Method
- Reaction mixture
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- Enzyme
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- 0.05 - 0.1 mg/ml solution
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- 1ml
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- Substrate
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- Brewer's yeast cell suspension
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- (2 mg dry weight/ml)
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- 3ml
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- Buffer
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- M/15 Phosphate buffer, pH 7.5
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- 5ml
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- Distilled water
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- 1ml
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- Total Volume
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- 10ml
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- Procedure
- After incubation for 2 hours at 25oC with gentle shaking, A800 of the mixture is determined. As a reference, 1 ml of distilled water is used instead of enzyme solution.
- Calculation
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- Percentage decrease in A800 =
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- (A800 of reference - A800 of reaction mixture)
x 100/intial A800 of reference |
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- When 60% of A800 decrease, equivalent to 2 units, is observed in the reaction system, the brewer's yeast cells are completely lysed, namely, 1 unit of Zymolyase® - 20T lyses 3 mg dry weight of brewer's yeast.
- PRECAUTIONS ON USE:
- Use a sterilized filter except nitrocellulose when a sterilized enzyme solution is needed.
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- STORAGE:
- Stable for at least 1 year at 2oC. About 70% of the lytic activity is lost when stored at 30oC for 3 months.
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