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| 人绒毛膜肿瘤细胞系 Bewo |
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CCL-98 人绒毛膜肿瘤细胞系 Bewo
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CCL-98™ |
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BeWo |
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RA Pattillo |
| Biosafety Level: |
1 |
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frozen |
| Medium & Serum: |
See Propagation |
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adherent |
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Homo sapiens (human) |
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epithelial
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Organ: placenta
Disease: choriocarcinoma |
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hormones; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); estrogen; estrone; estriol; estradiol; keratin |
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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| Related Cell Culture Products |
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Human poliovirus 3
Vesicular stomatitis virus |
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negative |
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Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9,11
D16S539: 13,14
D5S818: 10,11
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 16 |
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modal number = 86; range = 71 to 178.
Stemline number is hypotetraploid. Karyotype relatively stable within stemline number. |
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G6PD, B |
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male |
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C |
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Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: 3 to 4 times per week |
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Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020 |
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1219: Pattillo RA, et al. Human hormone production in vitro. Science 159: 1467-1469, 1968. PubMed: 5753554
22139: Pattillo RA, et al. Control mechanisms for gonadotrophic hormone production in vitro. In Vitro 6: 205-214, 1970. PubMed: 5535575
22343: Pattillo RA, et al. Estrogen production by trophoblastic tumors in tissue culture. J. Clin. Endocrinol. Metab. 34: 59-61, 1972. PubMed: 4332667
26271: Pattillo RA, Gey GO. The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro. Cancer Res. 28: 1231-1236, 1968. PubMed: 4299001
26272: Hertz R. Choriocarcinoma of women maintained in serial passage in hamster and rat. Proc. Soc. Exp. Biol. Med. 102: 77-81, 1959. PubMed: 14401422
26273: Pattillo RA, et al. The hormone-synthesizing trophoblastic cell in vitro: a model for cancer research and placental hormone synthesis. Ann. N.Y. Acad. Sci. 172: 288-298, 1971. PubMed: 5289994
32279: Schar BK, et al. Simultaneous detection of all four alkaline phosphatase isoenzymes in human germ cell tumors using reverse transcription-PCR. Cancer Res. 57: 3841-3846, 1997. PubMed: 9288797
32858: Heckert LL, et al. The cAMP response elements of the alpha subunit gene bind similar proteins in trophoblasts and gonadotropes but have distinct functional sequence requirements. J. Biol. Chem. 49: 31650-31656, 1996. PubMed: 8940185 | |
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