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猴EB病毒转化细胞系B95-8  

VR-1492  EB病毒转化细胞系 B95-8

ATCC® Number: VR-1492™    
Classification: Herpesviridae, Gammaherpesvirinae, Lymphocryptovirus
Agent: Human herpesvirus 4
Strain: B95-8
Original Source: human lymphoblastoid cell line 883L
Produced In: Human herpesvirus 4
Depositors: G Miller
Biosafety Level: 2 Shipped: frozen 5 x 1 ml.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Incubation : Duration:
Effect : Yes, in vitro effects: transforms/immortalizes primate B-lymphocytes
Store at : -70C or lower
Comments : VR-1492 is the designation for the ATCC set of five 1 ml vials of VR-1491.
As described by Caputo et al. (1991), a 1-ml aliquot of this EBV preparation is capable of transforming B lymphocytes from a preparation of normal human lymphocytes.
VR-1491 is the virus shed in the supernate of persistantly infected and transformed B95-8 cells (CRL-1612). The EBV virus itself is of human origin.
As noted by Tumilowicz et al. (1984), the B95-8 cells used to produce this strain of EBV are infected with and may also shed an uncharacterized type D retrovirus.
-70C or lower
host cell line, CRL-1612
Related Products: source culture:ATCC CRL-1612
multi-vial package:ATCC VR-1491
References : 22383: Caputo JL, et al. An effective method for establishing Human B lymphoblastic cell lines using Epstein-Barr Virus. J. Tissue Culture Methods 13: 39-44, 1991.
22670: Tumilowicz JJ, et al. Presence of retrovirus in the B95-8 Epstein-Barr virus-producing cell line from different sources. In Vitro 20: 486-492, 1984. PubMed: 6086497
26110: Miller G, et al. Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Proc. Natl. Acad. Sci. USA 69: 383-387, 1972. PubMed: 4333982

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